Part:BBa_K4170004:Design

Lbucas13a assembly part 3

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 2
  Illegal BsaI.rc site found at 1332
  

Design Notes

This part derives from pGJK_His-SUMO-LbuCas13a plasmid (Plasmid #172488) by mutagenesis PCR to efficiently mutate illegal restriction sites which hinder compatibility with RFC [10] iGEM standard. In addition, the primers exploited incorporate BsaI recognition sites external of the amplified sequence to allow for efficient Golden Gate assembly with the parts 1,2 and 4 along with the linearilized plasmid backbone

Source

This part derives from pGJK_His-SUMO-LbuCas13a plasmid (Plasmid #172488).